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    內蒙古農業大學:干酪乳桿菌Zhang在腸道內的“變化”

    發布日期:2021-07-26
      1.  基于宏基因組和宏轉錄組探究干酪乳桿菌Zhang(LcZ)在體內外的基因表達差異,以及腸道共生菌群對LcZ的應答;

      2.  LcZ進入人體腸道后,其復制和轉錄增加,而腸內原有的干酪乳桿菌菌株不受影響;

      3.  進入腸道后,LcZ約39%的mRNA和45%的sRNA被抑制,而rli28c sRNA被激活,其在體外抑制了LcZ生長和乳酸產生

      4.  LcZ的ABC轉運蛋白和氨基酸代謝基因、糖和SCFA代謝基因,分別在攝入第14和28天被激活;

      5.  人腸道菌群對LcZ的應答是有限且異質的。
     
    Metagenomic and metatranscriptomic profiling of Lactobacillus casei Zhang in the human gut
    人腸道中干酪乳桿菌Zhang的宏基因組和宏轉錄組分析
    10.1038/s41522-021-00227-2
    07-01, Article

    Abstract:
    Little is known about the replication and dynamic transcription of probiotics during their "passenger" journey in the human GI tract, which has therefore limited the understanding of their probiotic mechanisms. Here, metagenomic and metatranscriptomic sequencing was used to expose the in vivo expression patterns of the probiotic Lactobacillus casei Zhang (LcZ), which was compared with its in vitro growth transcriptomes, as well as the dynamics of the indigenous microbiome response to probiotic consumption. Extraction of the strain-specific reads revealed that replication and transcripts from the ingested LcZ were increased, while those from the resident L. casei strains remained unchanged. Mapping of all sequencing reads to LcZ genome showed that gene expression in vitro and in vivo differed dramatically. Approximately 39% of mRNAs and 45% of sRNAs of LcZ well-expressed were repressed after ingestion into human gut. The expression of ABC transporter genes and amino acid metabolism genes was induced at day 14 of ingestion, and genes for sugar and SCFA metabolism were activated at day 28 of ingestion. Expression of rli28c sRNA with peaked expression during the in vitro stationary phase was also activated in the human gut; this sRNA repressed LcZ growth and lactic acid production in vitro. However, the response of the human gut microbiome to LcZ was limited and heterogeneous. These findings implicate the ingested probiotic has to change its transcription patterns to survive and adapt in the human gut, and the time-dependent activation patterns indicate highly dynamic cross-talk between the probiotic and human gut microbes.

    First Authors:
    Jicheng Wang,Jiachao Zhang
    Correspondence Authors:
    Zhihong Sun
    All Authors:
    Jicheng Wang,Jiachao Zhang,Wenjun Liu,Heping Zhang,Zhihong Sun


      來源:網絡

     
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